It might not be overt, but that exasperation is picked up with all the sharp sensitivity of someone who's learned to track other things' bad moods for his own safety. Optimus is in no way any of the scientists he's used to dealing with, but there's a slight wince regardless. "I'm sorry. You're the first .. autonomous nonorganic being I've encountered, and all of this is very new to me."
And then Optimus becomes a vehicle, and he's forced to re-assess whether or not all these other trucks really are hidden, injured allies and the robot simply doesn't want to betray them to unknowns. Although there's visible surprise, he's still not afraid. "Please avoid coming in contact with my people specifically in that case, I'm afraid it wouldn't go well. They might try to take you apart so they can make their own versions." Scarlett would have a FIELD DAY.
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And then Optimus becomes a vehicle, and he's forced to re-assess whether or not all these other trucks really are hidden, injured allies and the robot simply doesn't want to betray them to unknowns. Although there's visible surprise, he's still not afraid. "Please avoid coming in contact with my people specifically in that case, I'm afraid it wouldn't go well. They might try to take you apart so they can make their own versions." Scarlett would have a FIELD DAY.